Occurrence of 5'-Deoxyadenosylcobalamin and Its Physiological Function as the Coenzyme of Methylmalonyl-CoA Mutase in a Marine Eukaryotic Microorganism, Schizochytrium limacinum SR21

Emi MIYAMOTO; Yuri TANIOKA; Tsugiyo YUKINO; Masahiro HAYASHI; Fumio WATANABE; Yoshihisa NAKANO

doi:10.3177/jnsv.53.471

Regular Paper

Occurrence of 5'-Deoxyadenosylcobalamin and Its Physiological Function as the Coenzyme of Methylmalonyl-CoA Mutase in a Marine Eukaryotic Microorganism, Schizochytrium limacinum SR21

Emi MIYAMOTO, Yuri TANIOKA, Tsugiyo YUKINO, Masahiro HAYASHI, Fumio WATANABE, Yoshihisa NAKANO

著者情報

キーワード: cobalamin, 5'-deoxyadenosylcobalamin, vitamin B₁₂, methylmalonyl-CoA mutase, Schizochytrium limacinum SR21

ジャーナルフリー

2007 年 53 巻 6 号 p. 471-475

DOI https://doi.org/10.3177/jnsv.53.471

詳細

抄録

A marine eukaryotic microorganism, Schizochytrium limacinum SR21, had the ability to absorb and accumulate exogenous cobalamin, which was converted to the cobalamin coenzymes 5'-deoxyadenosylcobalamin (20.1%) and methylcobalamin (29.6%). A considerably high activity (about 38 mU/mg protein) of 5'-deoxyadenosylcobalamin-dependent methylmalonyl-CoA mutase (EC 5.4.99.2) involved in amino acid and odd-chain fatty acid metabolism was found in the cell homogenate of S. limacinum SR21. The enzyme was purified to homogeneity and characterized.

責任著者(Corresponding author)

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