Transport of Vitamin B₆ in Human Erythrocytes

II. Effects of Incubation Temperature, Glycolytic Inhibitor and Vitamin B₆ Analogues on Transport of Vitamin B₆ in Human Erythrocytes

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Lowering incubation temperature resulted in a marked decreased transport of pyridoxine and pyridoxamine and a slightly decreased one of pyridoxal. Potassium fluoride inhibited the transport of pyridoxine, pyridoxamine and pyridoxal phosphate into the cells by 20, 15 and 60%, respectively, whereas the transport of pyridoxal was not inhibited. The conversion of pyridoxal to pyridoxal phosphate in the cells was also inhibited by the addition of potassium fluoride.
In the presence of deoxypyridoxine, the transport of pyridoxine and pyridoxamine into the cells was inhibited, while that of pyridoxal and pyridoxal phosphate was not. Further, pyridoxal phosphate concentrations in the cells after incubation with free forms of B₆ were decreased in the presence of deoxypyridoxine. Deoxypyridoxine phosphate did not inhibit the transport of various forms of B₆.
These evidences lead to the conclusion that the transport process of various forms of B₆ and their phosphorylation in erythrocytes are associated with the energy produced by intracellular glycolysis.