抄録
As a simple procedure for the separation of riboflavin tetrabutyrate and its hydrolysates, thin layer chromatography is suitable. To measure quantitatively the separated flavins, scratching out of the area of silica gel adsorbing flavin and direct application of the lumiflavin fluorescence method to the silica gel were examined. The results showed nearly 100% recovery, permitting application of the procedure of thin layer chromatography to the separating determination of synthetic riboflavin tetrabutyrate and riboflavin tetranicotinate and their partial hydrolysates as well as naturally, occurring flavins, free riboflavin, FMN and FAD.
