Structural and Immunochemical Homologies between Foxtail Millet Glutelin 60 kDa and Starch Granule-bound Starch Synthase Proteins from Rice, Barley, Corn and Wheat Grains

Kenji TAKUMI; Junko UDAKA; Masumi KIMOTO; Tetsuzo KOGA; Hideaki TSUJI

doi:10.3177/jnsv.46.109

Kenji TAKUMI, Junko UDAKA, Masumi KIMOTO, Tetsuzo KOGA, Hideaki TSUJI

著者情報

キーワード: foxtail millet, glutelin 60 kDa, N-terminal amino acid sequence, starch gran-ule-bound starch synthase, antigenic homology

ジャーナルフリー

2000 年 46 巻 2 号 p. 109-112

DOI https://doi.org/10.3177/jnsv.46.109

詳細

抄録

Foxtail millet glutelin 60 kDa (MG60) was purified by preparative SDS-PAGE, and the N-terminal amino acid sequence was determined within 20 residues. The result demonstrated that the primary structure at N-terminal of MG60 was almost identical to those of the granule-bound starch synthase (GBSS) proteins from rice, barley, corn, wheat and potato. The existence of common epitopes among MG60 and GBSS proteins from these starch-storing cereals were corroborated by immunoblot analysis using antisera raised against MG60. These facts strongly suggest a close relationship between MG60-like glutelins and GBSS proteins.

責任著者(Corresponding author)

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