Effect of serine protease trypsin on the interaction of RANKL and RANK
2019 年 53 巻 1 号 p. 9-14
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2019 年 53 巻 1 号 p. 9-14
Recent reports suggest that trypsin and the trypsin‐like enzyme tryptase modulate the ability of macrophages to polarize to the M2a phenotype. Trypsin also potentiates monocytes to differentiate into fibrocytes resulting in enhanced wound healing. Because osteoclasts differentiate from monocyte/macrophage lineage cells, the enzymatic activity might also affect osteoclast differentiation. In this study we examined the effect of trypsin on the ability of the mouse macrophage cell line RAW264.7 to differentiate into osteoclasts. We found that trypsin treatment of RAW264.7 cells lowers their ability to differentiate into osteoclasts in response to RANKL. We also found that trypsin treatment lowered the capacity of RANK to bind its cognate ligand RANKL. Therefore, we concluded that the exposure to trypsin or trypsin‐like enzymes might affect macrophage polarization to osteoclasts via degradation of the cell surface receptor RANK. (J Osaka Dent Univ 2019; 53: 9‐14)
