抄録
A gas chromatographic equilibration method to determine quantitatively organic solvents dissolved in blood was described. The method is characterized by the fact that when a quantity of organic solvent dissolved in a blood sample is equilibrated with a volume of overlying air, the amount of solvent vapor dissolved in the blood will be calculated from the concentration in the gas phase. In the experiment, one ml of standard material prepared akin to the sample was injected into a vial or syringe, and was equilibrated at 37°C in a thermo-regulated bath. After the equilibration had been established, one ml of overlying air was submitted to gas chromatographic analysis. As a result of investigations of various factors which influence the gas-blood partition coefficient, the method was proved to be able to separate and determine 0.02μg/ml of benzene, 0.04μg/ml of toluene, and 0.1μg/ml of m-xylene, the values corresponding to 1 cm of peak height on the chromatogram. The availability of the method was verified by an experiment in which five men were exposed for two hours to 25 ppm of benzene, 100 ppm of toluene, and 150 ppm of m-xylene.
