尿中NRK49-F細胞増殖促進活性の各種腎疾患の病態における役割
1994 年 106 巻 7-8 号 p. 875-882
詳細
1994 年 106 巻 7-8 号 p. 875-882
To evaluate the roles of transforming growth factor-β (TGF-β) on the glomerular cell proliferation and sclerosis, a new colorimetric bioassay system for urinary excretion of TGF-β has been developed by using NRK49-F cell culture. The urinary TGF-β activity was detected by this method in focal glomerular sclerosis (FGS) and purpura nephritis but not in minimal change nephrotic syndrome and IgA nephropathy. The bioassayed TGF-β activity was confirmed by a blocking experiment using a neutralizing antibody and by Western immunoblotting. These findings suggest that TGF-β plays an important role in the progression of the glomerular lesions, in FGS and purpura nephritis.
