Abstract
Isolation of two new strains of pseudomonas with characteristic surface-active properties were isolated from soil contaminated with natural surfactant. Observation of surface tension properties indicated that biosurfactants were produced by these newly isolated and promising strains of Pseudomonas sp. (SB 3 and SB 5). The isolation of the microbes, their harvesting in the nutrient media, extraction of the bacterial lipid and composition of those lipids extracted, are discussed in the present study.