Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Biochemistry & Biotechnology
Butyrate-Induced Growth Inhibition and Differentiation in Caco-2 Cells with Hybrid Liposomes
Zakir HOSSAINHideyuki KURIHARAMasashi HOSOKAWAKoretaro TAKAHASHI
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JOURNALS FREE ACCESS

2005 Volume 54 Issue 5 Pages 281-288

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Abstract

Hybrid liposomes were prepared by mixing glycerophospholipids with glyceroglycolipids. The effects of hybrid liposomes on Caco-2 cells were investigated. Growth inhibition of cells was determined with sodium 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium dye reduction assay. Alkaline phosphatase activity was measured for assessing the cell differentiation induced by sodium butyrate (NaBT). Growth of Caco-2 cells was inhibited at 48 h after addition of all four types hybrid liposomes (PC/DGDG, PC/SQDG, PS/DGDG and PS/SQDG) while at 72 h growth inhibition was significant for all treatments except the hybrid liposome of phosphatidylcholine and digalactosyldiacylglycerol under concentrations of 50 μM glycerophospholipids with 50 μM glyceroglycolipids. Alkaline phosphatase activity of Caco-2 cells increased significantly when cells were treated in combination of hybrid liposomes and NaBT at 72 h, indicating that hybrid liposomes enhanced cell differentiation induced with NaBT.

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© 2005 by Japan Oil Chemists' Society
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