α-Phenyl-N-tert-butyl Nitrone Has Scavenging Activity Against Singlet Oxygen (¹O₂) and Attenuates ¹O₂-Induced Neuronal Cell Death

抄録

Scavenging activity of α-phenyl-N-tert-butyl nitrone (PBN) against singlet oxygen (¹O₂) and its effects on ¹O₂-induced neuronal cell death were examined. PBN at 1 – 4 mM dose-dependently suppressed ¹O₂ released from activated human neutrophils. PBN did not react with hydrogen peroxide or hypochlorite and did not affect myeloperoxidase activity, which are involved in the ¹O₂ formation in neutrophils. PBN also suppressed chemically generated ¹O₂ in a cell-free system. These findings collectively indicated that PBN certainly has scavenging activity against ¹O₂. Furthermore, PBN attenuated ¹O₂-induced neuronal cell death. The well-known neuroprotective effects of PBN might be attributed to its ¹O₂-scavenging activity.