Effect of Cigarette Smoke Components on Vascular Smooth Muscle Cell Migration Toward Platelet-Derived Growth Factor BB

Shinji Yoshiyama; Takahiro Horinouchi; Soichi Miwa; Hong Hui Wang; Kazuhiro Kohama; Akio Nakamura

doi:10.1254/jphs.10283SC

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Effect of Cigarette Smoke Components on Vascular Smooth Muscle Cell Migration Toward Platelet-Derived Growth Factor BB

Shinji Yoshiyama, Takahiro Horinouchi, Soichi Miwa, Hong Hui Wang, Kazuhiro Kohama, Akio Nakamura

著者情報

キーワード: smooth muscle, nicotine, cigarette smoke extract (CSE)

ジャーナルフリー早期公開

論文ID: 10283SC

DOI https://doi.org/10.1254/jphs.10283SC

この記事には本公開記事があります。

The final version of this article is now available: Vol. 115 (2011) , No. 4 p.532

詳細

抄録

Cigarette smoking is one of the factors causing accumulation of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques. Changes in cell migration toward platelet-derived growth factor BB were investigated using a Boyden chamber after 48-h preincubation of GBaSM-4 VSMCs with nicotine or nicotine-free cigarette smoke extract (CSE). A nicotine concentration of 0.1 μM maximally promoted cell migration; 0.1% CSE also promoted cell migration, while high CSE concentrations damaged GBaSM-4 cells. Fetal bovine serum (FBS) long-depletion induced decrease in migration of GBaSM-4 cells. Our results suggest that nicotine and some CSE components can induce GBaSM-4 cell migration.

責任著者(Corresponding author)

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