Morphological changes of liver cell membranes in CCl4-administered rats were observed electronmicroscopically by using mainly a negative staining method. CCl4 caused not only disappearance of elementary particles attached to the surface of cell membranes, but also that of hexagonal subunit patterns (treated with 1% DOC) in the membranes of tight junction 3-6 hr after administration. Present data indicates that CCl4 primarily affects the plasma membrane rather than endoplasmic reticulum.
