Anodized and hydrothermally treated titanium with a nanotopographic structure combined with dental pulp stem cells promotes bone formation in a rat calvarial bone defect model

Toshiyuki Kobayashi; Masaki Hata; Yoshihiko Sugita; Atsushi Aoyagi; Ryohei Matsukawa; Yuka Imanishi; Takeshi Hagio; Kensuke Kuroda; Hatsuhiko Maeda; Jun Takebe

doi:10.2186/jpr.JPR_D_24_00176

Toshiyuki Kobayashi, Masaki Hata, Yoshihiko Sugita, Atsushi Aoyagi, Ryohei Matsukawa, Yuka Imanishi, Takeshi Hagio, Kensuke Kuroda, Hatsuhiko Maeda, Jun Takebe

著者情報

Toshiyuki Kobayashi
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Masaki Hata
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Yoshihiko Sugita
Department of Oral Pathology/Forensic Odontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Atsushi Aoyagi
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Ryohei Matsukawa
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Yuka Imanishi
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Takeshi Hagio
Institute of Materials Innovation, Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan
Kensuke Kuroda
Institute of Materials Innovation, Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan
Hatsuhiko Maeda
Department of Oral Pathology/Forensic Odontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan
Jun Takebe 責任著者
Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Japan

キーワード: Discharge anodic oxidation and hydrothermal treatment, Nanotopography, Dental pulp stem cells, Transplantation

ジャーナルオープンアクセス早期公開

論文ID: JPR_D_24_00176

DOI https://doi.org/10.2186/jpr.JPR_D_24_00176

詳細

抄録

Purpose: Spark-discharged anodic oxidation coating on commercially pure titanium (SAc.p.Ti) has been shown to promote bone conduction and bone matrix mineralization during new bone formation. This study hypothesized that the combination of SAc.p.Ti with dental pulp stem cells (DPSCs) would enhance new bone formation. The objective was to evaluate the effect of this combination in a rat bone defect model.

Methods: DPSCs were isolated from Sprague–Dawley (SD) rat incisors and cultured. Calvarial bone defects were created in SD rats, followed by transplantation of commercially pure titanium (c.p.Ti), SAc.p.Ti, or SAc.p.Ti combined with DPSCs. Bone formation was assessed using micro-computed tomography (micro-CT). Toluidine blue O staining was employed to evaluate bone-implant contact and the newly formed bone area. Hematoxylin–eosin (HE) staining was performed to identify osteoblast-like cells.

Results: Micro-CT analysis revealed hard tissue formation on the surface of SAc.p.Ti. Toluidine blue O staining showed significantly greater bone-implant contact and newly formed bone area in the SAc.p.Ti/DPSC group compared to the c.p.Ti and SAc.p.Ti groups. HE staining confirmed the presence of osteoblast-like cells at the defect margins, with evidence of new bone formation on the surface of SAc.p.Ti and in the SAc.p.Ti/DPSC groups.

Conclusions: The combination of SAc.p.Ti and DPSCs presents a promising strategy for promoting new bone formation in rat calvarial defect model.

責任著者(Corresponding author)

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