2011 Volume 48 Issue 1 Pages 73-77
The aim of this study was to examine the changes in the density of immunoreactive avian β-defensins (ir-avβDs) -3 and -11 in response to lipopolysaccharide (LPS). Healthy laying hens were intravenously injected with LPS (1mg/kg of body weight) at 0, 3, 6 and 12h (n=4) before tissue collection, which was uniformly 6h after oviposition. Their uterus tissues were fixed in formalin-PBS and processed for paraffin sections. Immunohistochemistry was then performed using polyclonal antibodies for avβD-3 and -11. Immunoreactive avβD-3 was localized in the supranuclear cytoplasm of the surface epithelial cells, whereas ir-avβD-11 was observed in the apical cytoplasm of those cells. The density of the immunoreaction products of avβD-3 showed a significant decrease at 3h after LPS injection compared to 0h group, and then returned to the original level by 6h. On the other hand, the density of the immunoreaction products of avβD-11 was significantly higher at 3h and 12h after LPS injection compared to the 0h group. These results suggest that avβD-3 protein was secreted, and avβD-11 was synthesized and stored in the mucosal epithelial cells in response to LPS injection. These two types of avβDs may play a significant role in the innate host defense in the hen uterus.