乳酸菌と酵母菌の共培養上清の局所投与は、上皮性ケラチノサイトによるサイトカイン産生抑制を介して、アトピー性皮膚炎モデルマウスにおける炎症反応を有意に改善した。

抄録

Approximately 10% of dogs suffer from atopic dermatitis (AD) and long-term treatment for AD impacts pet owners both physically and economically. In this study, we focused on the co-culture supernatant from Lactobacillus and Saccharomyces (LS) as new probiotics and examined the efficacy of LS on the development of AD. Anti-inflammatory effect of LS was confirmed using human epithelial keratinocytes (HaCaT). Pro-inflammatory cytokines production by stimulated HaCaT treated with LS were measured. Anti-allergic properties of topical treatment of LS were examined in a mouse model of AD. After AD symptoms were developed, daily topical treatment of LS was performed for 3 weeks. Clinical symptoms were monitored weekly, and immune responses were analyzed. Secretion of IL-6, IL-8, TARC and TNFα by stimulated-HaCaT was significantly decreased by co-cultured with LS. Whereas no significant change of clinical symptom was observed by LS treatment, allergy-related immune reactions including the number of IgE-positive B cells and activated dendritic cells in the local lymph nodes was significantly decreased in the LS treatment group. Our findings suggest that LS has supplemental therapeutic effects on the AD via direct inhibition of inflammatory cytokines released from epithelial keratinocytes.