Factors Affecting Fertilization and Embryonic Development During Intracytoplasmic Sperm Injection in Pigs

Michiko NAKAI; Naomi KASHIWAZAKI; Junya ITO; Naoki MAEDOMARI; Manabu OZAWA; Masao SHINO; Junko NOGUCHI; Hiroyuki KANEKO; Kazuhiro KIKUCHI

doi:10.1262/jrd.10-200E

SRD Young Investigator Award

Factors Affecting Fertilization and Embryonic Development During Intracytoplasmic Sperm Injection in Pigs

Michiko NAKAI, Naomi KASHIWAZAKI, Junya ITO, Naoki MAEDOMARI, Manabu OZAWA, Masao SHINO, Junko NOGUCHI, Hiroyuki KANEKO, Kazuhiro KIKUCHI

著者情報

キーワード: Embryonic development, Fertilization, ICSI, Pig

ジャーナルフリー

2011 年 57 巻 2 号 p. 183-187

DOI https://doi.org/10.1262/jrd.10-200E

詳細

抄録

In intracytoplasmic sperm injection (ICSI) technique, a sperm was injected into ooplasm directly using a glass pipette. The fertilization physiology in ICSI is considered quite different from that of the natural fertilization. The different mechanisms for fertilization may be the causes of various results in ICSI. In this paper, we focus on the state of sperm membranes, nuclear or DNA integrity during ICSI procedure and discuss the influence of these factors on fertilization and embryonic development. We also introduce some examples in application of ICSI for new technologies in pigs.

責任著者(Corresponding author)

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