Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles

Ahmad Farid RAWAN; Hikmatullah LANGAR; Maho MUNETOMO; Yuki YAMAMOTO; Kohei KAWANO; Koji KIMURA

doi:10.1262/jrd.2023-047

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Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles

Ahmad Farid RAWAN, Hikmatullah LANGAR, Maho MUNETOMO, Yuki YAMAMOTO, Kohei KAWANO, Koji KIMURA

著者情報

Ahmad Farid RAWAN
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Pre-Clinic Department, Veterinary Science Faculty, Nangarhar University, 2603, Afghanistan
Hikmatullah LANGAR
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Maho MUNETOMO
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Yuki YAMAMOTO
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-0054, Japan
Kohei KAWANO
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
Koji KIMURA
Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan

キーワード: Estradiol receptor, Follicle, Insulin-like growth factor-1 (IGF-1), Steroidogenic enzymes

ジャーナルオープンアクセス早期公開

論文ID: 2023-047

DOI https://doi.org/10.1262/jrd.2023-047

この記事には本公開記事があります。

The final version of this article is now available: Vol. 69 (2023), No. 6 pp. 337-346

詳細

抄録

Insulin-like growth factor-1 (IGF-1) plays a crucial role in follicular growth and stimulates steroid hormone production in bovine follicles. Steroid hormones are synthesized through the actions of steroidogenic enzymes, specifically STAR, CYP11A1, HSD3B, and CYP19A1 in both theca cells (TCs) and granulosa cells (GCs), under the influence of gonadotropins. Particularly, estradiol 17β (E2) assumes a central role in follicular development and selection by activating estrogen receptors β (ESR2) in GCs. We assessed ESR2 mRNA expression in GCs of developing follicles and investigated the impact of IGF-1 on the mRNA expression of ESR2, CYP19A1, FSHR, and LHCGR, STAR, CYP11A1, and HSD17B in cultured GCs and TCs, respectively.Additionally, we assessed the influence of IGF-1 on androstenedione (A4), progesterone (P4), and testosterone (T) production in TCs. Small-sized follicles (< 6 mm) exhibited the highest levels of ESR2 mRNA expression, whereas medium-sized follicles (7–8 mm) displayed higher levels than large-sized follicles (≥ 9 mm) (P < 0.05). IGF-1 increased the mRNA expression of ESR2, CYP19A1, and FSHR in GCs of follicles of both sizes, except for FSHR mRNA in medium-sized follicles (P < 0.05). IGF-1 significantly elevated mRNA expression of LHCGR, STAR, CYP11A1, and CYP17B in TCs of small- and medium-sized follicles (P < 0.05). Moreover, IGF-1 augmented the production of A4 and P4 but had no impact on T production in TCs of small- and medium-sized follicles. Taken together, our findings indicate that IGF-1 upregulates steroidogenic enzymes and steroid hormone production, underscoring the crucial role of IGF-1 in follicle development and selection.

Graphical Abstract Fullsize Image

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