Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment

Kotaro HORIGUCHI; Takehiro TSUKADA; Saishu YOSHIDA; Ken FUJIWARA; Takashi NAKAKURA; Morio AZUMA; Ayano SHINDO; Rumi HASEGAWA; Shu TAKIGAMI

doi:10.1262/jrd.2024-033

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Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment

Kotaro HORIGUCHI, Takehiro TSUKADA, Saishu YOSHIDA, Ken FUJIWARA, Takashi NAKAKURA, Morio AZUMA, Ayano SHINDO, Rumi HASEGAWA, Shu TAKIGAMI

著者情報

キーワード: CD9, Hanging drop three-dimensional cell culture, Intermediate lobe, Pituitary gland, Stem cells

ジャーナルオープンアクセス早期公開

論文ID: 2024-033

DOI https://doi.org/10.1262/jrd.2024-033

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2024), No. 5 pp. 343-347

詳細

抄録

The adenohypophysis is composed of the anterior and intermediate lobes (AL and IL, respectively), and secretes hormones that play an important role in reproduction. CD9- and SOX2-double (CD9/SOX2) positive cells located in the marginal cell layer (MCL) facing the Rathke’s cleft in the AL and IL form the primary stem cell niche in the adult adenohypophysis of rats. In this study, we successfully obtained 3-dimensional (3D) cell aggregates that closely resembled the primary niche of MCL in vivo. After incubation in a Matrigel containing several growth factors, approximately 20% of the cells in the CD9/SOX2-positive cell aggregates were differentiated into hormone-producing cells. The cell aggregates generated in this study may provide insight into the regulation of the pituitary stem/progenitor cell niche and the turnover of hormone-producing cells.

Graphical Abstract Fullsize Image

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