抄録
Quantitative test for the determination of individual PMSG in the pregnant mare is one of the important tools for pregnancy diagnosis. The present paper reports on the quantitative assay of standard PMSG and pregnant mare sera using antibody agar plate method. The research using antibody agar plate were performed according to modification of Fafey's technique3). With this technique, specific anti-PMSG immuno rabbit serum (IgG) is mixed uniformly in an agar plate. Antigen containing solutions are placed in small antigen wells cut in the agar. A concentric ring of antigen-antibody reaction forms around the antigen well. By graphically comparing the ring diameters with those of appropriate standard, the concentration of the test sera of pregnant mares can be determined. The mean PMSG levels in 30 pregnant mare sera were found to be minimum 60 IU/ml and maximum 210 IU/ml from pregnant mare sera. However, for definite evalution of the samples, more experimental data will be needed. In this study, by means of the antibody agar plate technique, it was possible to use the anti-PMSG serum for an immunoassay for qua, titative analysis.
