Gonadotrophin による排卵誘起反応における皮下,腹腔内及び静脈注射の鏡敏度の比較

I. 成熟雌ラットに対する HCG および HCG と下垂体末混合物の投与成績

抄録

Since, it was difficult to understand that the sensitivity of the rat for inducing ovulation by the single subcutaneous injection of HCG is about equal to that of the rabbit in Friedman's test, the effect of route of administration on induced ovulation in adult rats was studied.
An improved method for intravenous injection of adult rats is devised to eliminate the inhibitory effect of anesthetization on the induction of ovulation⁴⁾. The injection is performed safely under unane-sthetized condition without fastening the animal. The rat is took up gently from its back by a hand of the assistant. The head and one of forelimbs of the rat are held by the ring formed by the thumb and the forefinger and the other forelimb is held between the fore-and middlefingers of the hand. The animal is stood on its rump in the other palm of the assistant and the legs and the tail are held by fingers of the hand. The one side leg is held by the thumb and the forefinger. The vena metatarsea dorsalis pedis may be elevated by applying pressure to the saphenous vein by the ball of the forefinger or the tip of the thumb. The paw is held by the left hand of the operator and the hormone is injected into the metatarsal vein by 1/4 or 1/5 needle for blood vessel. This technique is also applied to obtain the blood.
The response of rats to induce ovulation by the subcutaneous injection of HCG or the combined pre-paration of 9 parts of HCG and 1 part of pituitary powder was slightly less sensitive than Friedman's test. By the intraperitoneal injection, The ovulating response by HCG was 3 time sensitive than the cases by subcutaneous injection. The most sensitive route to induce ovulation by HCG or by the com-bined preparation was the intravenous injection, which was 7 or more times as sensitive as the subc-utaneous injection and about 2 or 5 times as sensitive as the intraperitoneal administration or Friedman's test respectively. These results may furnish some information for clinical application of gonadotrophins. In the test of induced ovulation in rats, the animals are autopsied in the afternoon of the day of vag-inal proestrus, when the uterus is distended with fluid in intact animals. However, in ovulation induced animals, it was observed a tendency to decrease the uterine weight and uterine fluid.