The Base Sequence Corresponding to DYZ1 is Detectable in the DNA from Many Animal Species
1992 年 38 巻 1 号 p. 55-59
詳細
1992 年 38 巻 1 号 p. 55-59
Primers possessing base sequences complementary to the 3512-3534th base (Y1.1) and 78-100th base (Y1.2) of a specific repeat base sequence (DYZ1) on the long of the human Y chromosome were prepared. Using these primers, 1μg of cellular DNA from humans, cattle, pigs, rats and mice (both sexes) and testis DNA from salmon and herring were amplified by Polymerase Chain Reaction. In the case of human cellular DNA, the appearance of amplified DNA fragments (154 bp) following electrophoresis, and 55C and 65C hybridization, was noted only for male DNA. In the case of bovine, swine, rat and mouse DNA, such amplified DNA fragments were seen in both male and female DNA after 55C and 65C hybridization. The DNA fragments were also detected in salmon and herring DNA after 55C hybridization. These results suggest that the DYZ1 region is present in the DNA from many mammalian speces and some fishes but also on an autosome in various mammals with the exception of humans.
