抄録
The semen collection process in the boar is far from being a sterile procedure. Consequently, freshly collected boar ejaculates commonly contain bacterial contamination. The negative effects of bacteria contamination appear to be concentration-dependent, affecting both in vitro quality and longevity of extended semen.
The aim of this study was to identify the bacteria in boar semen during the semen collection and the liquid preservation process and to find the resistance of bacteria to antibiotics.
Twelve boars (Duroc) originating from four AI center were used. Each boar was collected three times with gloved-hand technique. Semen was diluted with Androhep (without antibiotics) with 3*107/ml and stored at 5 and 17°C. The ejaculates were also checked for the following bacterial contamination. All strains grew on sheep blood agar and the colonies were tested for Gram stain, catalase and oxidase activity. Bacteria were identified by automated instrument for rapid organism identification system and 8 antibiotics (amikasin, polymyxin B, neomycin, streptomycin, kanamycin, penicillin, erythromycin, and tetracycline) sensitivities were performed using the Kirby-Bauer Disk Diffusion.
The Bacterial contaminants of Staphylococcus genus (37.8%), Proteu genus (7.0%), Bacillus genus (6.1%), Pasteulla genus (5.7%), Acinetobacte genus (5.2%), Serratia genus (4.3%) and other pathogens (33.9%) were frequently isolated. Antibiotic sensitivity was higher in amicasin than other antibiotics.
(Key words: boar, semen, bacterial contamination)
