Studies on the factors influencing superovulation and MII oocyte recovery in cynomolgus monkey

抄録

Non-human primates have been widely used for biomedical researches; however, relatively a little have been studied regarding assisted reproduction technologies (ARTs) including oocyte recovery and in vitro maturation (IVM). Thus, the current study was carried out to investigate the factors influencing superovulation and IVM. Female cynomolgus monkeys (40∼70 month-aged) were superovulated by sequential injection with leuplin (3.7 mg/head, once), human follicle stimulating hormone (75 IU/kg, 3 times, 3 days interval), and human chorionic gonadotropin (hCG; 1,200 IU/head, once). Oocytes were surgically recovered from ovaries, and immature oocytes were cultured in the presence of various concentrations (0, 1, and 10 IU/ml) of pregnant mares serum gonadotropin (PMSG) and hCG. Interestingly, MII oocyte recovery rate was increased in female age- and body weight-dependent manners, and optimal age and body weight for MII oocyte recovery appeared to be 52 month and 3.13 kg, respectively. In addition, most notable increase in IVM rate (62.5%) was found in the use of 5 IU/ml PMSG and hCG compared to control and 10 IU/ml treatment groups (32.0% and 34.8%, respectively). From these findings, we suggest that basic experimental conditions for superovulation and MII oocyte recovery were established in cynomolgus monkeys.