抄録
The daf-16 gene encodes a forkhead transcription factor that presumably regulates the downstream target genes in an insulin-like signal pathway. It is still unknown what genes are regulated by DAF-16, but at least some genes related anti-oxidation are expected, because daf-2 mutants are resistant to oxidative stress and a daf-16 mutation suppresses this phenotype. The daf-16 mutant itself is also sensitive to oxygen. We examined what anti-oxidant enzymes are regulated by DAF-16. Furthermore, we analyzed a transcriptional profiling of daf-16(m26) allele using an expressed sequence tag (EST) microarray experiments to detect the target genes of DAF-16. As the result, the change of the expression level of a few anti-oxidant genes was found in daf-16(m26) mutants, e.g., the levels of a microsomal catalase (ctl-2), a metallothionein gene (mtl-1) and lysosomal protease genes were decreased. These suggest that anti-oxidant proteins are strong candidates in the downstream target genes regulated by the DAF-16 transcription factor. [J Radiat Res 44:390 (2003)]
