抄録
Genomic DNA of acute myeloid leukemia (AML) cells induced by x-ray in C3H/He mice is frequently accompanied with illegitimate insertion of the cDNA of the intracisternal A-particle (IAP) DNA element that is present in normal murine genome in two thousand copies per haploid and is closely related with provirus form of retrovirus in the structure. We established the PCR-based method to detect the characteristic integration site of IAP cDNA in tumors. From genomic DNAs of 11 lines of radiation-induced AML cells from C3H/He mice, 21 sites of the unique integration sites of IAP cDNA were amplified by this method. In contrast, occurrence of only 4 insertion of IAP cDNA were newly observed among 40 lines of thymic lymphoma cells from C3H/He mice. Since insertion of IAP element with H-type LTR was observed in all the characteristic site, suggesting that activation of the H-type LTR of IAP is common feature in hematopoietic tumors in C3H/He mice. [J Radiat Res 44:395 (2003)]
