Transgenerational Mutations in the Children of the Liquidators Who Worked for the Chernobyl Nuclear Power Plant Accident

抄録

To examine the transgenerational mutations by ionising radiation in human, microsatellite length polymorphic mutations have been investigated in the F1 offspring of the liquidators who worked for the Chernobyl accident. Sixty four exposed families (liquidators living in Minsk after the accident) and 67 unexposed families living in Minsk were examined. DNA were extracted from the peripheral lymphocytes (father, mother and children) and used for amplifying microsatellites by PCR. Mutations were detected using automated sequencer with Gene Scan program. Five and four mutations were found in the 61 (69 F1) exposed and 23 (25 F1) unexposed families, respectively, using 15 microsatellites linked with X- and autosomes. Only one mutation was found at two Y linked microsatellites among 39 exposed families (42 sons) and none were found in 13 unexposed families (13 sons). Significant differences are not found between exposed and unexposed groups at present. [J Radiat Res 44:420 (2003)]