抄録
In order to clear the characteristics of heavy-ion beams cells, the p53 induction by heavy-ion beam irradiation was compared to the results from reference irradiation with -rays. As well known, p53 is important protein at cell-cycle control or apoptosis in response to DNA damage or other stress. Recently protein CARF is identified as a protein having deep relation to p53. In this study, there is another interest on the interaction of p53 and CARF when the cells are irradiated with heavy-ion beams. U2OS cells were irradiated with 220 MeV 20Ne7+ beams at the beam line of TIARA AVF-cyclotron. The value of LET of the beam was 430 keV/m. As a reference experiment, UV irradiation was done with a germicidal lamp. Irradiated samples were kept in incubator for various hours after irradiation and analyzed, such as immunostainig, Western blotting and immunoprecipitation. The induction of p53 was clearly observed in both cases of irradiation with Ne-ion beams and UV. The amount of p53 induction was the maximum at 24 hours after irradiation. On the other hand, the induction of protein CARF was not clear in the case of irradiation with Ne-ion beams, while it was clearly shown with UV irradiation. Furthermore, the agreement of the localization of p53 and CARF was observed undoubtedly in the case of irradiation with ultraviolet, though the co-localization of these proteins was not seen in the case of irradiation with Ne-ion beams. Some differences in the interaction of p53 to CARF may exist between the case of irradiation with Ne-ion beams and UV.
