Direct hemoperfusion using activated charcoal was performed in patients with hepatic coma. The charcoal used was extracted, and was fractionated into organic, aqueous phases. The organic phase was analysed by a gas chromatography-mass spectrometer. The analysis give 168 peaks from which 31 substances were identified. The aqueous phase was used for amino acid, amine and middle molecular analyses. Amine was fractionated on Dowex 50w x8 column and analysed by gas chromatography-mass spectrometer. Tyramine, spermine, spermidine, putrescine and 46 other amine peaks were detected. In the middle molecular analysis, the fractions, a and b, obtained by gel chromatography on Sephadex G-15 were further analysed by reversed-phase chromatography on μ Bondapak C18 column. 50 peaks were detected by UV absorption at 280nm and about 25 peptide peaks, by fluorometric detection.
