Prevention of Shear-induced Platelet Aggregation by Immobilized Apyrase

Yoshiharu MIURA; Sadayoshi AOYAGI; Kouichi HIROTA; Toshiko FUJINO; Fujiko KITAHORI; Kazuhisa MIYAMOTO

doi:10.11392/jsao1972.10.997

Yoshiharu MIURA, Sadayoshi AOYAGI, Kouichi HIROTA, Toshiko FUJINO, Fujiko KITAHORI, Kazuhisa MIYAMOTO

著者情報

キーワード: Apyrase, Immobilization of enzyme, ADP-induced platelet aggregation, Antithrombogenic biomaterial

ジャーナルフリー

1981 年 10 巻 6 号 p. 997-1000

DOI https://doi.org/10.11392/jsao1972.10.997

詳細

抄録

In a shear field, platelet aggregation was induced by ADP liberated from injured erythrocytes, and thereby free platelet counts were significantly decreased in rabbit whole blood. The enzymatic elimination of inducer ADP was effective for the prevention of platelet aggregation. For this purpose, apyrase was immobilized on Sepharose 4B, nylon, and ethylene-vinyl alcohol copolymer. Immobilized preparations of apyras e could effectively decompose ADP and thus prevent platelet aggregation.

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