LPS固定化ビーズにおける抗腫瘍活性

阿部 元; 谷 徹; 沼 謙司; 遠藤 善裕; 吉岡 豊一; 青木 裕彦; 松田 孝一; 花沢 一芳; 石井 豊; 蔦本 慶裕; 小玉 正智

doi:10.11392/jsao1972.20.230

TRIAL OF ANTICANCER EFFECTS USING LPS IMMOBILIZED BEADS

H ABE, T TANI, K NUMA, Y ENDO, T YOSHIOKA, H AOKI, K MATSUDA, K HANASAWA, Y ISHII, Y TSUTAMOTO, M KODAMA

Author information

H ABE
First Dept. of Surgery, Shiga University of Medical Science
T TANI
First Dept. of Surgery, Shiga University of Medical Science
K NUMA
First Dept. of Surgery, Shiga University of Medical Science
Y ENDO
First Dept. of Surgery, Shiga University of Medical Science
T YOSHIOKA
First Dept. of Surgery, Shiga University of Medical Science
H AOKI
First Dept. of Surgery, Shiga University of Medical Science
K MATSUDA
First Dept. of Surgery, Shiga University of Medical Science
K HANASAWA
First Dept. of Surgery, Shiga University of Medical Science
Y ISHII
First Dept. of Surgery, Shiga University of Medical Science
Y TSUTAMOTO
First Dept. of Surgery, Shiga University of Medical Science
M KODAMA
First Dept. of Surgery, Shiga University of Medical Science

Keywords: lipopolysaccharide(LPS), polystyrene beads, cytotoxicity, direct hemoperfusion, immunomodulation

JOURNAL FREE ACCESS

1991 Volume 20 Issue 1 Pages 230-234

DOI https://doi.org/10.11392/jsao1972.20.230

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Abstract

Lipopolysaccharide(LPS) and its components were immobilized to polystyrene beads in order to utilize its anti-tumor effect without toxicity. Spleen cells from C3H/HeN mice and SD rats were activated by contact stimulation with immobilized beads and cytotoxicity tests were measured by ⁵¹Cr release assay. The beads immobilizing a component of LPS enhanced little cytotoxicity. Activated spleen cells from mice with E. coli LPS immobilized beads led to strong cytotoxicity, and those from rats with Salmonella LPS immobilized beads led to strong cytotoxicity. Suppression of tumor growth and lung metastasis was recognized after activated spleen cells injection. Direct hemoperfusion with Salmonella LPS immobilized beads was safely carried out in rats.

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