抄録
Mycobacterium tuberculosis and Mycobacterium bovis were differentiated from other mycobacteria by their negative growth on salicylate-Ogawa medium and on nitrate medium. These two organisms could be separated by the niacin test, nicotinamidase test, and susceptibility to thiophene-2-carbonic acid hydrazide (TCH). M. tuberculosis showed a positive niacin test, showed a positive nicotinamidase activity, and grew on the TCH medium, whereas M. bovis showed a negative niacin test, showed a negative nicotinamidase activity, and did not grow on the TCH medium. If used singly, these three reactions had shown a few exceptions, but these exceptions were excluded by the combined use of the three methods. Thus, M. tuberculosis and M. bovis seemed to be identified by the above five in vitro tests. Remark: Salicylate-Ogawa medium-Ogawa egg medium containing 0.5mg/ml and 1.0mg/ml sodium salicylate. Nitrate medium-a modified Sauton agar medium, in which sodium nitrate was substituted for asparagine. TCH medium-Ogawa egg medium containing 10μg/ml and 50μg/ml TCH.
