Studies on Yersinia enterocolitica
I. Isolation of Antigenic Fractions from Y. enterocolitica
1976 Volume 31 Issue 2 Pages 301-307
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1976 Volume 31 Issue 2 Pages 301-307
To clarify the cellular components of Yersinia enterocolitica related to toxicity and antigenic characteristics, studies were made on toxicity and chemical and antigenic characteristics of the saline extract (Fr. I) and hot phenol-water extract (Fr. II) of the bacterial cells, as well as of sugar components (Fr. III) obtained by hydrolysis of Fr. II with 0.1 N acetic acid.
When determined by thin-layer chromatography and liquid chromatography the sugar composition was essentially the same among Fr. I, II, and III. It consisted of galactose, glucose, xylose, and an unidentified sugar that appeared to be 6-deoxy-L-altrose reported by Ellwood and Kirk.
In the double gel-diffusion precipitation test against anti- Y. enterocolitica whole cell antiserum, Fr. I, II, and III formed one fused precipitation line, and Fr. I and II an additional precipitation line. Even heating at 100°C for 1 hour or treatment with chloroform did not alter the precipitation pattern of Fr. I.
When 250 μg (as sugars) of Fr. II was injected intravenously into 5 mice, 2 mice died within 72 hours. When 5 mice were injected with 1, 500 μg of Fr. II, all of them died. In contrast, no mice died when injected intravenously with 2, 000 μg of Fr. III.
