Nippon Saikingaku Zasshi

[title in Japanese]

[in Japanese]

1976 Volume 31 Issue 2 Pages 285-299
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.285

JOURNAL FREE ACCESS

Download PDF (2384K)
Studies on Yersinia enterocolitica

I. Isolation of Antigenic Fractions from Y. enterocolitica

Shigeru INO, Fumihisa SAKUMA, Mitsuru USUYAMA

1976 Volume 31 Issue 2 Pages 301-307
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.301

JOURNAL FREE ACCESS

Show abstractHide abstract

To clarify the cellular components of Yersinia enterocolitica related to toxicity and antigenic characteristics, studies were made on toxicity and chemical and antigenic characteristics of the saline extract (Fr. I) and hot phenol-water extract (Fr. II) of the bacterial cells, as well as of sugar components (Fr. III) obtained by hydrolysis of Fr. II with 0.1 N acetic acid.
When determined by thin-layer chromatography and liquid chromatography the sugar composition was essentially the same among Fr. I, II, and III. It consisted of galactose, glucose, xylose, and an unidentified sugar that appeared to be 6-deoxy-L-altrose reported by Ellwood and Kirk.
In the double gel-diffusion precipitation test against anti- Y. enterocolitica whole cell antiserum, Fr. I, II, and III formed one fused precipitation line, and Fr. I and II an additional precipitation line. Even heating at 100°C for 1 hour or treatment with chloroform did not alter the precipitation pattern of Fr. I.
When 250 μg (as sugars) of Fr. II was injected intravenously into 5 mice, 2 mice died within 72 hours. When 5 mice were injected with 1, 500 μg of Fr. II, all of them died. In contrast, no mice died when injected intravenously with 2, 000 μg of Fr. III.

View full abstract

Download PDF (6385K)
Population Changes of T-and B-Lymphocytes in the Spleen of Mice after Infection with BCG, Salmonella typhimurium TV 148 or Staphylococcus aureus 209 P

Hiroaki ASOU, Tatsuo SAITO, Hideko HORI, Yoshiko TSURUMI, Masao J. TAN ...

1976 Volume 31 Issue 2 Pages 309-315
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.309

JOURNAL FREE ACCESS

Show abstractHide abstract

The populations of T- and B-lymphocytes in the spleen were examined in mice of the AKR strain by the cytotoxicity test with rabbit anti-mouse-specific bone marrow-derived lymphocyte antigen serum or rabbit anti-mouse thymocyte serum plus complement. Some of these mice had been injected intravenously with live organisms of BCG, Salmonella ty-phimurium TV148, or Staphylococcus aureus 209 P, and others with heat-killed organisms of BCG or TV148. Those two prepared antisera had been confirmed in several ways to have specific cytotoxicity against T- and B-lymphocytes, respectively, in the presence of complement.
The T cell population in the spleen showed a slight decrease in mice in the early stage of infection with 1× 10⁷ organisms of BCG, but a slight increase in the same mice 3 to 5 weeks later. During the experimental period of infection with BCG no obvious change could be found in the B cell population in the spleen in these mice. A significant increase of the T cell population in the spleen was also observed in mice which were infected with 1× 10³ to 1× 10⁷ organisms of TV 148 3 weeks before. The B cell population in the spleen, however, decreased gradually in these mice after infection. Mice injected with heat-killed organisms of BCG or TV148, instead of live organisms, showed no increase of the T cell population in the spleen. In mice infected with 2× 10⁸ organisms of 209P, there was a slight decrease of both T- and B-lymphocyte populations in the spleen. This result suggested an expansion of the population of some other cells in the spleen.

View full abstract

Download PDF (1246K)
A Study on Purification of Staphylococcal Enterotoxin A

Yoshimi SHIBATA, Morihiro MORITA, Yasuji AMANO, Nakao ISHIDA

1976 Volume 31 Issue 2 Pages 317-322
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.317

JOURNAL FREE ACCESS

Show abstractHide abstract

Staphylococcal enterotoxin A was isolated in a highly purified form by means of ionexchange resin adsorption, gel filtration, trypsinization, and ultrafiltration. Amberlite CG-50 ion-exchange resin adsorption is a convenient way to concentrate crude toxin from a large volume of culture fluid. Ultrafiltration with Amicon UM-10 is another useful means to concentrate. The molecular sieve is an efficient tool to concentrate the toxin from diluted fluid. When used in an insolubilized form, trypsin was proved to be significantly effective for eliminating α-hemolysin selectively from crude enterotoxin A. The purified sample appeared to be a single entity when examined by immunoelectrophoresis and CM-cellulose column chromatography.
The specific activity of the purified sample was 356 times as high as that of the starting material when determined on protein concentration. The yield of the sample was 40% of the starting material.

View full abstract

Download PDF (2892K)
[title in Japanese]

1976 Volume 31 Issue 2 Pages 323-330
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.323

JOURNAL FREE ACCESS

Download PDF (1283K)
[title in Japanese]

1976 Volume 31 Issue 2 Pages 331-344
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.331

JOURNAL FREE ACCESS

Download PDF (2824K)
[title in Japanese]

1976 Volume 31 Issue 2 Pages 345-350
Published: March 25, 1976
Released on J-STAGE: June 17, 2011

DOIhttps://doi.org/10.3412/jsb.31.345

JOURNAL FREE ACCESS

Download PDF (971K)

Register with J-STAGE for free!