Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
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Easy sectioning of whole grain of rice using cryomicrotome
Wan-Yi ChiouKazuo TsuganeTadafumi KawamotoMasahiko Maekawa
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2018 Volume 68 Issue 3 Pages 381-384

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Abstract

To obtain a clear intact section of a ripened rice grain, which is suitable for biochemical and histological analysis, the Kawamoto method using a specific adhesive film was applied using a cryomicrotome. The longitudinal and sagittal sections were easily obtained together with the cross-section, and cell characteristics were clearly discerned in the ripened grain. It was demonstrated that the Kawamoto method is readily applicable for intact sectioning of hard tissue, including ripened grain. Intact section sampling may be useful for enzymatic analysis and transcriptomic analysis of plant tissue.

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© 2018 by JAPANESE SOCIETY OF BREEDING
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