A new enzymatic method is presented for the determination of serum free fatty acids (FFA), utilizing acyl-CoA synthetase (ACS), acyl-CoA oxidase (ACO) and peroxidase.
The standard curve for the method is linear for FFA concentrations up to 2,000μmol/l.Within-run and between-run precision studies showed CV´s of≤1 and ≤ 2%, respectively.
lnterference by bilirubin and lipemia are eliminated by use of potaSsium ferrocyanide and lipoProtein liPase resPectively.
