Solid phase immunoassay of corticosterone was established using fluorescent tag labelled 2nd antibody and corticosterone conjugated polyacrylamide beads (lmmunobead®).The sensitivity of this assay was 40pg/tube.Light scattering from the beads was negligible in this assay conditbns.The most suitable protein to protect non-specific binding of materials to the assay tubes was highly purified gelatin because of its lower intensity of the back ground signal.
