Abstract
We developed a method for measuring urinary 17 ketosteroids (17 KS) without prior hydrolysis of its conjugated forms. Both free and conjugated forms of 17 KS were successfully extracted by passing the samle through a BSA-ODS minicolumn (TSK), eluting with 80% methanol and drying. After the Zimmermann reaction, the Zimmermann chromogen was extracted with 1-butanol. Our proposed method gave rapid results for urinary 17 KS and showed good reproducibility (C. V.: 2.41-8.75%), recovery (about 100%) and correlation with the conventional method for determining 17 KS (r=0.881, a regression line, y=0.565x+4.52, n=25).
