臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
市販のIgGを用いたEnd Point法によるヒト血清中の抗体濃度の測定
宮川 誠鶴藤 真室田 哲郎吉川 邦衛佐藤 茂
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1989 年 18 巻 3 号 p. 124-129

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Specific antibody concentration in human sera was determined by the end point method using double antibody solid phase radioimmunoassay (SP-RIA). The concentration of standard IgG at the end point was measured with sandwitch SP-RIA, and used as standards for determining antibody concentratlons in human sera. Error extents of the sensitivity of double antibody SP-RIA showed within 10-fold ranges, and its minimum detection levels gave the values from 1.42 to 4.25ng/ml. When the SP-RIA was applied to determine human antibody, 11 of 102 samples were judged as anti-FBS antibody positive samples. The positive distribution ranges revealed the values from 50.5 to 455.5ng/ml of anti-FBSantibody concentrations.
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