抄録
We assayed three different parameters of fibrin and/or fibrinogen degradation products (FDP), i. e. FDP-T, FDP-E, and FDP-DD, using an automated latex photometric immunoassay (LPIA) system with latex reagents conjugated with anti-fibrinogen, anti-fibrinogen-E domain, and anti-neoantigen of fibrinogen-D domain antibody, respectively. Although correlation coefficients between each pair were statistically significant, several samples showed discrepancies among the three FDP parameters. In order to test the possibility that these discrepancies might be caused by the difference in the affinity of the three antibodies to each FDP fragment, we analyzed the profiles of FDP fragments by Western blotting. Consequently, we found abundant high-molecular-weight (HMW) and/or D fragments in some samples which showed low FDP-DD and FDP-E values relative to FDP-T values. Generally, FDP-DD is considered to be the most reliable parameter for monitoring fibrinolysis. However, our study indicated that FDP-T is remarkably useful whenever FDP-DD values are lower than expected from the clinical parameters and/or other laboratory test data used for diagnosis of DIC.
