植物の生長調節
Online ISSN : 2189-6305
Print ISSN : 1346-5406
技術ノート
植物における一過的タンパク質大量発現系「つくばシステム」について
三浦 謙治
著者情報
ジャーナル 認証あり

2019 年 54 巻 1 号 p. 77-81

詳細
抄録

To produce recombinant proteins and identify the gene functions, such as subcellular localization, one of the strongest agro-infiltration-based transient protein expression system for plant cells, termed ‘Tsukuba system’ has been established. By using this system, about 4 mg/g fresh weight of protein expression was detected in Nicotiana benthamiana. Furthermore, the system is also applicable to various plant species, including eggplant, tomato cultivars and wild species, lettuce, hot pepper, soybean, common bean, and orchid. The vector pBYR2HS contains a geminiviral replication system and a double terminator. Thus, the system is an effective and valuable tool for rapid transient expression to obtain a bunch of proteins and to examine gene function.

著者関連情報
© 2019 一般社団法人植物化学調節学会
前の記事 次の記事
feedback
Top