オオヤマザクラのクローン間差が組織培養における生産コストに及ぼす影響

抄録

Tissue culture costs of 3 superior wild clones of Prunus sargentii Rehder (PS 1, PS 2, PS 3) for the Japanese traditional handicraft industry was studied using sprout axillary buds. The overall degree of micropropagation difficulty of the 3 clones was in the order of PS 3, PS 2 and PS 1 judging from shoot propagation, rooting, plug and acclimation. The estimated cost of the plug was 304 yen (PS 1), 485-646 yen (PS 2) and 509-680 yen (PS 3). PS 1 was propagated with1 or 3.16μM BAP, but PS 2 and PS 3 only propagated with 1μM BAP. Under shoot propagation with 1μM BAP, the rooting was only observed in PS 2. Rooting and shoot development under rooting conditions indicated different patterns of IBA response among 3 clones. The clonal differences of phytohormonal response among 3 clones are considered to be due to the physiological status and/or genetic background.