Abstract
We examined whether or not some protease inhibitors prevent endothelial cell injury due to PMA-activated leukocytes. The effects of protease inhibitors on the cytotoxicity were determined by measuring the release of 51Cr from prelabeled cultured endothelial cells. We also examined the leukocyte produced active oxygen spPcics by measuring luminol-chenliluminescence.
Among the protease inhibitors tested, aprotinin and urinastatin decreased the endothelial cell injury in a dose dependent manner.
These data suggest that the protease inhibitors suppress priming effect of the leukocyte produced proteases on the endothelial cell injury induced by active oxygen species.
