抄録
We have examined the mechanism of peptide leukotriene (LT) production in rat liver. LTC4 synthesizing activity was shown to be localized in hepatocyte rather than non-parenchymal cells including Kupffer cell. Stimulation of the isolated hepatocyte with calcium ionophore (A 23187) did not cause any production of peptide LTs, whereas addition of LTA4induced a remarkable production of peptide LTs without the A 23187 stimulation. Kupffer cell which was shown to generate only a slight amount of peptide LTs with the A 23187 stimulation even in the presence of LTA4, produced remarkably peptide LTs when LTC4synthase partially purified from the hepatocyte was included into the culture system. Coculture of hepatocyte markedly enhanced production of peptide LTs.
These results indicate that an enzymatic cooperation between hepatocyte and Kupffer cell would play an important role in generating peptide LTs in rat liver.
