Detection of novel and recurrent conjoined genes in non-Hodgkin B-cell     lymphoma

Yosuke Matsumoto; Taku Tsukamoto; Yoshiaki Chinen; Yuji Shimura; Nana Sasaki; Hisao Nagoshi; Ryuichi Sato; Hiroko Adachi; Masakazu Nakano; Shigeo Horiike; Junya Kuroda; Tomohiko Taki; Kei Tashiro; Masafumi Taniwaki

doi:10.3960/jslrt.20033

Original Article

Detection of novel and recurrent conjoined genes in non-Hodgkin B-cell lymphoma

Yosuke Matsumoto , Taku Tsukamoto, Yoshiaki Chinen, Yuji Shimura, Nana Sasaki, Hisao Nagoshi, Ryuichi Sato, Hiroko Adachi, Masakazu Nakano, Shigeo Horiike, Junya Kuroda, Tomohiko Taki, Kei Tashiro, Masafumi Taniwaki

著者情報

Yosuke Matsumoto 責任著者
Department of Hematology, Japanese Red Cross Kyoto Daiichi Hospital, Kyoto, Japan,
Taku Tsukamoto
Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Yoshiaki Chinen
Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Department of Hematology, Fukuchiyama City Hospital, Fukuchiyama, Japan,
Yuji Shimura
Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Nana Sasaki
Department of Hematology, Japanese Red Cross Kyoto Daini Hospital, Kyoto, Japan,
Hisao Nagoshi
Department of Hematology and Oncology, Hiroshima University, Hiroshima, Japan,
Ryuichi Sato
Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Hiroko Adachi
Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Masakazu Nakano
Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Shigeo Horiike
Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Junya Kuroda
Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Tomohiko Taki
Department of Medical Technology, Kyorin University Faculty of Health Science, Tokyo, Japan,
Kei Tashiro
Department of Genomic Medical Sciences, Kyoto Prefectural University of Medicine, Kyoto, Japan,
Masafumi Taniwaki
Center for Molecular Diagnostics and Therapeutics, Kyoto Prefectural University of Medicine, Kyoto, Japan.

キーワード: Conjoined genes, LARP1 , MRPL2 , non-Hodgkin B-cell lymphoma

ジャーナルオープンアクセス
電子付録

2021 年 61 巻 2 号 p. 71-77

DOI https://doi.org/10.3960/jslrt.20033

Browse “Advance online publication” version

詳細

抄録

For this study, we investigated comprehensive expression of conjoined genes (CGs) in non-Hodgkin B-cell lymphoma (B-NHL) cell line KPUM-UH1 by using paired-end RNA sequencing. Furthermore, we analyzed the expression of these transcripts in an additional 21 cell lines, 37 primary samples of various malignancies and peripheral blood mononuclear cells of four normal individuals. Seventeen CGs were detected in KPUM-UH1: CTBS-GNG5, SRP9-EPHX1, RMND5A-ANAPC, OTX1-EHBP1, ATF2-CHN1, PRKAA1-TTC33, LARP1-MRPL22, LOC105379697-BAK1, TIAM2-SCAF8, SPAG1-VPS13B, WBP1L-CNNM2, NARS2-GAB2, CTSC-RAB38, VAMP1-CD27-AS1, LRRC37A2-NSF, UBA2-WTIP and ZNF600-ZNF611. To our knowledge, 10 of these genes have not been previously reported. The various characteristics of the CGs included in- and out-of-frame fusions, chimeras involving non-coding RNA and transcript variants. A finding of note was that LARP1-MRPL2 was characterized as in-frame fusion and was recurrently expressed in B-NHL samples. In this study, variety of CGs was expressed both in malignant and normal cells, some of which might be specific to lymphoma.

責任著者(Corresponding author)

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