The cellular uptake of adriamycin (ADM) and hematoporphyrin derivative (HpD) Was investigated using a fluorescence spectrophotometer and KK-47 cells in vitro. Three types of combination of ADM and HpD,(1) ADM→HpD,(2)HpD→ADM, and (3) ADM+HpD, were studied in terms of cellular uptake and phototoxicity. The HpD uptake was large in order of (3)>(1)>(2). The cytotoxicity of ADM was thought to be inhibited by cellularly incorporated HpD in order of (3)>(1)>(2). When the cells treated with the 3 combination procedures were irradiated with an argon laser (488.0 and 514.5nm) or argon-dye laser (635+5 nm), the degree of cytotoxicity was large in order of (1)>(3)>(2). Particularly, with the argon laser, the degree was in order of (1)=(3)>(2).
