抄録
The effect of low power He-Ne laser (632.8 nm, 8.5 mW) on hypotonic hemolysis in human red cells was investigated.
Red cell floating saline solutions (0.8% NaCl, pH7.0) were centrifuged and the sediments were irradiated for 0 (control), 1, 3, 5, 7, 10, 15 and 30 minutes. Each irradiated sample was transfered into a hypotonic solution (0.405%NaCl, pH 7.0 ). After leaving the samples at room temperature for 10 minutes, optical density of every suspension was measured spectrophotomechanically at 545 nm of wave length to determine hemoglobin content.
Percent hemolysis of the control samples was 51.7±10.3% (mean±SD, n=18). Relative hemolysis at 10 and 15-minute irradiation significantly declined in comparison to the control value.
These results suggest that low power laser beams have a protective effect on red cells from hypotonic hemolysis.
