The reaction of super pulse laser, which has very high peak power and very short pulse duration, in living cell destruction was investigated.
Pulse YAG laser with Q switch, which is able to oscillate three different wavelengths, was used for the super pulse laser. Its pulse duration is 8 nanosecond and its original wavelength is 1,064nm which is involved in infra red. The second harmonics oscillates 532nm as visible laser. The third harmonics oscillate 355nm as ultra violet laser. Rat and rabbit were anesthetized and super pulse laser was irradiated to there muscle for 10 minutes with one pulse/second and 46-1,000mJ/cm2 of energy under three different wavelengths. The reaction in cell destruction was investigated with an electron microscope.
In 1,064nm, almost no reaction were observed. In 532nm, mitochondria was expanded. In 355nm, mitochondria was destructed to be. vacuous. The heat diffusion inside the cell is thought to be very small with super pulse laser irradiation, as the destructed particle inside the cell was able to be detected.
