Laser photoirradiation treatment using hematoporphyrin derivatives (HPD) has been successfully applied to treat breast cancer, bronchial cancer or other cacncers. However, there has been no report about its successful application in human giomas. We considered it mandatory to perform basic experiment before its application for human glioma.
Using rat T1 glioma inoculated subcuteneously, up-take of HPD was confirmed 24, 48, and 72 hours after intravenous injection of HPD.
Using HPD containing Ti cells cultured in vitro, laser irradiation of 630 nm, 50-400 mW and 15 minutes interval was shown to cause cell damage or cell death.
These data suggested that laser photoirradiation with HPD will be very effective for human gliomas. If some difficulties such as laser output wattages, penetration into tissues, price of laser machine, etc is overcome, this method of treatment will be one of the main therapeutic modalities for human gliomas.
