This investigation aimed to test the potential value of CO2 laser fusion for knot security, so as to cope with the slipping aid untying of the sutural knot, which may lead to undue postoperative complications. Knot pull slipping load was determined by utilizing an intron tensionmeter for two-square nylon monofilament suture knots. When compared to the non-lased knots, the optimally lased identical suture knots was judged to provide a significantly higher level of knot security end, thus, to be free from the disastrous knot failure. Some trials in human models suggested this technique may have important surgical applications in the future.