胆道閉塞時のCeruloplasmin代謝

抄録

It has been described that serum ceruloplasmin is markedly elevated in various biliary obstructive disorders, but the underlying mechanism is yet unknown in many respects. This investigation represents an attempt to clarify the mechanism from the viewpoint of biosynthesis of ceruloplasmin by experiments to rats with ligated biliary tracts.
After sample of blood were drawn to assay for serum ceruloplasmin, alkaline phosphattase and bilirubin, the rats with cholangioligation were given intravenous injection of ⁶⁴Cu and the consequent measurement of serum ⁶⁴Cu-ceruloplasmin level at regular intervals was carried out.
The results obtained were follows
1. Serum ceruloplasmin showed a sharp increase from 48 hours after bile tract ligation.
2. The elevation of serum cerulasmin in bile tract-ligated rats was noted to have positive correlation with increase of serum alkaline phosphatase and bilirubin.
3. ⁶⁴Cu uptake by the liver and kindney ligated rats did not differ significantly from those of the control.
4. Animals with cholangioligation displayed evidence of increased cerulopasmin biosynthesis in the liver at 48 and 72 hours after ligation.
Although the possibility of suppression of hepatic ceruloplasmin clearance (decomposition and excretion) cannot be ruled out as a contributing factor, the serum ceruloplasmin elevation observed in ligated rats seems to reflect chiefly increased biosynthesis of ceruloplasmin because there was neither evidence of significant dilution of injected isotope in the serum nor did the hepatic or renal ⁶⁴Cu uptake in ligated animal differ significantly from the controls.
The experiment in rats demonstrates elevation of serum ceruloplasmin following ligation of the biliary duct, as in patients with various biliary obstructive disorders who are known to show such increase of the ceruloplasmin. The experimental findings obtained suggest that the underlying mechanism consists in increased ceruloplasmin biosynthesis as is the rule with serum alkaline phosphatase.